Localization and Distribution of 'Candidatus Liberibacter asiaticus’ in Citrus and Periwinkle by Direct Tissue Blot Immuno Assay with an Anti-OmpA Polyclonal Antibody
Identifieur interne : 000560 ( Main/Exploration ); précédent : 000559; suivant : 000561Localization and Distribution of 'Candidatus Liberibacter asiaticus’ in Citrus and Periwinkle by Direct Tissue Blot Immuno Assay with an Anti-OmpA Polyclonal Antibody
Auteurs : Fang Ding [États-Unis, République populaire de Chine] ; Yongping Duan [États-Unis] ; Cristina Paul [États-Unis] ; Ronald H. Brlansky [États-Unis] ; John S. Hartung [États-Unis]Source :
- PLoS ONE [ 1932-6203 ] ; 2015.
English descriptors
- KwdEn :
- MESH :
- chemical , immunology : Antibodies, Bacterial, Bacterial Outer Membrane Proteins.
- isolation & purification : Helicobacter.
- microbiology : Citrus sinensis, Phloem, Plant Leaves, Seeds, Vinca.
- Immunoblotting.
Abstract
‘
Url:
DOI: 10.1371/journal.pone.0123939
PubMed: 25946013
PubMed Central: 4422590
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Localization and Distribution of '<italic>Candidatus</italic> Liberibacter asiaticus’ in Citrus and Periwinkle by Direct Tissue Blot Immuno Assay with an Anti-OmpA Polyclonal Antibody</title><author><name sortKey="Ding, Fang" sort="Ding, Fang" uniqKey="Ding F" first="Fang" last="Ding">Fang Ding</name><affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>USDA ARS Molecular Plant Pathology Laboratory, Beltsville, Maryland, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>USDA ARS Molecular Plant Pathology Laboratory, Beltsville, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, P. R. China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Plant Science and Technology, Huazhong Agricultural University, Wuhan</wicri:regionArea><wicri:noRegion>Wuhan</wicri:noRegion></affiliation></author><author><name sortKey="Duan, Yongping" sort="Duan, Yongping" uniqKey="Duan Y" first="Yongping" last="Duan">Yongping Duan</name><affiliation wicri:level="2"><nlm:aff id="aff003"><addr-line>USDA ARS Horticultural Research Laboratory, Fort Pierce, Florida, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>USDA ARS Horticultural Research Laboratory, Fort Pierce, Florida</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Paul, Cristina" sort="Paul, Cristina" uniqKey="Paul C" first="Cristina" last="Paul">Cristina Paul</name><affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>USDA ARS Molecular Plant Pathology Laboratory, Beltsville, Maryland, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>USDA ARS Molecular Plant Pathology Laboratory, Beltsville, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Brlansky, Ronald H" sort="Brlansky, Ronald H" uniqKey="Brlansky R" first="Ronald H." last="Brlansky">Ronald H. Brlansky</name><affiliation wicri:level="2"><nlm:aff id="aff004"><addr-line>University of Florida, citrus Research and Education Center, Lake Alfred, Florida, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>University of Florida, citrus Research and Education Center, Lake Alfred, Florida</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Hartung, John S" sort="Hartung, John S" uniqKey="Hartung J" first="John S." last="Hartung">John S. Hartung</name><affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>USDA ARS Molecular Plant Pathology Laboratory, Beltsville, Maryland, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>USDA ARS Molecular Plant Pathology Laboratory, Beltsville, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">25946013</idno><idno type="pmc">4422590</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4422590</idno><idno type="RBID">PMC:4422590</idno><idno type="doi">10.1371/journal.pone.0123939</idno><date when="2015">2015</date><idno type="wicri:Area/Pmc/Corpus">000143</idno><idno type="wicri:Area/Pmc/Curation">000143</idno><idno type="wicri:Area/Pmc/Checkpoint">000440</idno><idno type="wicri:source">PubMed</idno><idno type="wicri:Area/PubMed/Corpus">000178</idno><idno type="wicri:Area/PubMed/Curation">000178</idno><idno type="wicri:Area/PubMed/Checkpoint">000178</idno><idno type="wicri:Area/Ncbi/Merge">001B18</idno><idno type="wicri:Area/Ncbi/Curation">001B18</idno><idno type="wicri:Area/Ncbi/Checkpoint">001B18</idno><idno type="wicri:Area/Main/Merge">000560</idno><idno type="wicri:Area/Main/Curation">000560</idno><idno type="wicri:Area/Main/Exploration">000560</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Localization and Distribution of '<italic>Candidatus</italic> Liberibacter asiaticus’ in Citrus and Periwinkle by Direct Tissue Blot Immuno Assay with an Anti-OmpA Polyclonal Antibody</title><author><name sortKey="Ding, Fang" sort="Ding, Fang" uniqKey="Ding F" first="Fang" last="Ding">Fang Ding</name><affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>USDA ARS Molecular Plant Pathology Laboratory, Beltsville, Maryland, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>USDA ARS Molecular Plant Pathology Laboratory, Beltsville, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, P. R. China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Plant Science and Technology, Huazhong Agricultural University, Wuhan</wicri:regionArea><wicri:noRegion>Wuhan</wicri:noRegion></affiliation></author><author><name sortKey="Duan, Yongping" sort="Duan, Yongping" uniqKey="Duan Y" first="Yongping" last="Duan">Yongping Duan</name><affiliation wicri:level="2"><nlm:aff id="aff003"><addr-line>USDA ARS Horticultural Research Laboratory, Fort Pierce, Florida, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>USDA ARS Horticultural Research Laboratory, Fort Pierce, Florida</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Paul, Cristina" sort="Paul, Cristina" uniqKey="Paul C" first="Cristina" last="Paul">Cristina Paul</name><affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>USDA ARS Molecular Plant Pathology Laboratory, Beltsville, Maryland, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>USDA ARS Molecular Plant Pathology Laboratory, Beltsville, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Brlansky, Ronald H" sort="Brlansky, Ronald H" uniqKey="Brlansky R" first="Ronald H." last="Brlansky">Ronald H. Brlansky</name><affiliation wicri:level="2"><nlm:aff id="aff004"><addr-line>University of Florida, citrus Research and Education Center, Lake Alfred, Florida, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>University of Florida, citrus Research and Education Center, Lake Alfred, Florida</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Hartung, John S" sort="Hartung, John S" uniqKey="Hartung J" first="John S." last="Hartung">John S. Hartung</name><affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>USDA ARS Molecular Plant Pathology Laboratory, Beltsville, Maryland, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>USDA ARS Molecular Plant Pathology Laboratory, Beltsville, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Bacterial (immunology)</term><term>Bacterial Outer Membrane Proteins (immunology)</term><term>Citrus sinensis (microbiology)</term><term>Helicobacter (isolation & purification)</term><term>Immunoblotting</term><term>Phloem (microbiology)</term><term>Plant Leaves (microbiology)</term><term>Seeds (microbiology)</term><term>Vinca (microbiology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Bacterial</term><term>Bacterial Outer Membrane Proteins</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Helicobacter</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Citrus sinensis</term><term>Phloem</term><term>Plant Leaves</term><term>Seeds</term><term>Vinca</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Immunoblotting</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>‘<italic>Candidatus</italic> Liberibacter asiaticus’ (CaLas), a non-cultured member of the α-proteobacteria, is the causal agent of citrus Huanglongbing (HLB). Due to the difficulties of in vitro culture, antibodies against CaLas have not been widely used in studies of this pathogen. We have used an anti-OmpA polyclonal antibody based direct tissue blot immunoassay to localize CaLas in different citrus tissues and in periwinkle leaves. In citrus petioles, CaLas was unevenly distributed in the phloem sieve tubes, and tended to colonize in phloem sieve tubes on the underside of petioles in preference to the upper side of petioles. Both the leaf abscission zone and the junction of the petiole and leaf midrib had fewer CaLas bacteria compared to the main portions of the petiole and the midribs. Colonies of CaLas in phloem sieve tubes were more frequently found in stems with symptomatic leaves than in stems with asymptomatic leaves with an uneven distribution pattern. In serial sections taken from the receptacle to the peduncle, more CaLas were observed in the peduncle sections adjacent to the stem. In seed, CaLas was located in the seed coat. Many fewer CaLas were found in the roots, as compared to the seeds and petioles when samples were collected from trees with obvious foliar symptoms. The direct tissue blot immuno assay was adapted to whole periwinkle leaves infected by CaLas. The pathogen was distributed throughout the lateral veins and the results were correlated with results of qPCR. Our data provide direct spatial and anatomical information for CaLas in planta. This simple and scalable method may facilitate the future research on the interaction of CaLas and host plant.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Gottwald, Tr" uniqKey="Gottwald T">TR Gottwald</name></author><author><name sortKey="Aubert, B" uniqKey="Aubert B">B Aubert</name></author><author><name sortKey="Zhao, Xy" uniqKey="Zhao X">XY Zhao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bove, Jm" uniqKey="Bove J">JM Bové</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Husain, Ma" uniqKey="Husain M">MA Husain</name></author><author><name sortKey="Nath, D" uniqKey="Nath D">D Nath</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Capoor, Sp" uniqKey="Capoor S">SP Capoor</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Hoffman, Mt" 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uniqKey="Cantisani M">M Cantisani</name></author><author><name sortKey="Tarallo, R" uniqKey="Tarallo R">R Tarallo</name></author><author><name sortKey="Della Pepa, Me" uniqKey="Della Pepa M">ME Della Pepa</name></author><author><name sortKey="D Oriano, V" uniqKey="D Oriano V">V D'Oriano</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>République populaire de Chine</li><li>États-Unis</li></country><region><li>Floride</li><li>Maryland</li></region></list><tree><country name="États-Unis"><region name="Maryland"><name sortKey="Ding, Fang" sort="Ding, Fang" uniqKey="Ding F" first="Fang" last="Ding">Fang Ding</name></region><name sortKey="Brlansky, Ronald H" sort="Brlansky, Ronald H" uniqKey="Brlansky R" first="Ronald H." last="Brlansky">Ronald H. Brlansky</name><name sortKey="Duan, Yongping" sort="Duan, Yongping" uniqKey="Duan Y" first="Yongping" last="Duan">Yongping Duan</name><name sortKey="Hartung, John S" sort="Hartung, John S" uniqKey="Hartung J" first="John S." last="Hartung">John S. Hartung</name><name sortKey="Paul, Cristina" sort="Paul, Cristina" uniqKey="Paul C" first="Cristina" last="Paul">Cristina Paul</name></country><country name="République populaire de Chine"><noRegion><name sortKey="Ding, Fang" sort="Ding, Fang" uniqKey="Ding F" first="Fang" last="Ding">Fang Ding</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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